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Expansión ex vivo de células T tumor-reactiva por medio de la 1/Ionomycin briostatina y la formulación de la cadena gamma común de citoquinas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Expansión ex vivo de células T tumor-reactiva por medio de la 1/Ionomycin briostatina y la formulación de la cadena gamma común de citoquinas

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14,615 Views

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07:20 min

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January 14, 2011

DOI:

10.3791/2381-v

DOI:
10.3791/2381-v

•

07:20 min
January 14, 2011

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1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Summary

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Un protocolo eficiente para la<em> Ex vivo</em> Expansión de las células T tumor-reactiva de los ganglios linfáticos que drenan tumores u otros tejidos linfoides secundarios, de los portadores de tumores anfitriones se describe. Este protocolo se expande de forma selectiva del tumor de células T específicas para el uso de la inmunoterapia adoptiva del cáncer de mama.

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Kmieciak, M., Toor, A., Graham, L., Bear, H. D., Manjili, M. H. Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation. J. Vis. Exp. (47), e2381, doi:10.3791/2381 (2011).

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