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Expansão ex vivo de células tumorais T-reativa por Meios de 1/Ionomycin Bryostatin eo Common Gamma Cadeia Formulação Citocinas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Expansão ex vivo de células tumorais T-reativa por Meios de 1/Ionomycin Bryostatin eo Common Gamma Cadeia Formulação Citocinas

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14,615 Views

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07:20 min

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January 14, 2011

DOI:

10.3791/2381-v

DOI:
10.3791/2381-v

•

07:20 min
January 14, 2011

•

1 Views
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1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Summary

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Um protocolo eficiente para a<em> Ex vivo</em> Expansão das células tumorais T-reativa a partir de nós tumor de drenagem linfáticos ou outros tecidos linfóides secundários de portadores de tumor hosts é descrito. Este protocolo seletivamente as células do tumor se expande T específicas para uso em imunoterapia adotiva de câncer de mama.

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Kmieciak, M., Toor, A., Graham, L., Bear, H. D., Manjili, M. H. Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation. J. Vis. Exp. (47), e2381, doi:10.3791/2381 (2011).

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