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配对补丁钳从电机神经元和斑马鱼的目标骨骼肌
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
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配对补丁钳从电机神经元和斑马鱼的目标骨骼肌

DOI:
10.3791/2351-v

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07:29 min

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November 20, 2010

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,
1Vollum Institute,Oregon Health and Sciences University

Chapters

  • 00:00Title
  • 01:21Preparation of Fish for Paired Recordings
  • 03:35Paired Recordings of CaP Motor Neuron and Target Muscle Cells
  • 06:41Conclusion

Summary

Automatic Translation

Automatic Translation

幼虫斑马鱼第一脊椎动物的模型系统，让从脊髓运动神经元和目标骨骼肌同时膜片钳记录。本视频演示了用于识别段第电机神经元和目标肌肉细胞以及从不同的细胞类型的录音方法的微观方法。

Tags

Patch Clamp RecordingMotor-neuronTarget MuscleZebrafish LarvaVertebrate Model SystemCaP Motor-neuronMorphologyLocationMicroscopic MethodsDye FillingAction Potential WaveformCell Input ResistanceLong-term RecordingsFrequency-dependence Of Synaptic TransmissionNeuromuscular JunctionQuantal SizeWhole Cell Voltage ClampUnitary EventsRelease PropertiesVesicle RecyclingSynaptic DepressionSynaptic FacilitationIn Vivo Preparation

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