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Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
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Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
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07:29 min
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November 20, 2010
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Summary
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Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.
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Cite this Article
Wen, H., Brehm, P. Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish. J. Vis. Exp. (45), e2351, doi:10.3791/2351 (2010).
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