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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

English

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12,136 Views

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09:40 min

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July 10, 2013

DOI:

10.3791/50543-v

DOI:
10.3791/50543-v

•

09:40 min
July 10, 2013

•

8 Views
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1Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Sciences,University of Miami

Summary

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We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.

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Aplysia CalifornicaSensory NeuronsPatch ClampGlutamatergic CurrentsMarine Gastropod MolluskNervous System FunctionLearning And MemoryNeuronal Co-cultureDissociated NeuronsIon CurrentsBiophysical CharacterizationPleural GangliaBuccal Ganglia

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Fieber, L. A., Carlson, S. L., Kempsell, A. T., Greer, J. B., Schmale, M. C. Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents. J. Vis. Exp. (77), e50543, doi:10.3791/50543 (2013).

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