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Transgenik kullanarak Assaying β-amiloid Toksisite C. elegans Model
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
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Transgenik kullanarak Assaying β-amiloid Toksisite C. elegans Model

DOI:
10.3791/2252-v

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13:59 min

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October 09, 2010

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1Institute for Behavioral Genetics,University of Colorado, 2Integrative Physiology,University of Colorado

Chapters

  • 00:00Title
  • 00:21Subscription Link
  • 02:52Introduction
  • 03:31Preparing Nematoda Growth Media Plates for Paralysis Assay
  • 05:34Preparation of Age-synchronous Worm Populations
  • 08:04Transgene Induction and Paralysis Assay
  • 11:48Representative Results
  • 13:05Conclusion

Summary

Automatic Translation

Automatic Translation

Yoğun çalışılan bir nematod solucanı<em> Caenorhabditis elegans</em> Transgenically insan β-amiloid peptid (Aβ) ifade etmek için tasarlanmış olabilir. Aβ, İndüklenmiş ifade<em> C. elegans</em> Kas hızlı, tekrarlanabilir felç fenotip Aβ toksisite modüle tedavileri izlemek için kullanılabilecek bir yol açar.

Tags

Assayingβ-amyloid ToxicityTransgenic C. Elegans ModelAlzheimer’s DiseaseToxicity MechanismInclusion Body MyositisNematode WormCaenorhabditis ElegansTransgenic EngineeringAβ ExpressionMeasurable PhenotypesAssociative LearningParalysis PhenotypeTemperature-sensitive MutationMRNA Surveillance SystemTemperature-inducible Muscle ExpressionAβ TransgeneProtective TransgeneGinkgo Biloba Extracts

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