Journal
/
Neuroscience
/
含量测定β-淀粉样蛋白的毒性，使用转基因 C。线虫模型
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
Please note that all translations are automatically generated. Click here for the English version.

含量测定β-淀粉样蛋白的毒性，使用转基因 C。线虫模型

DOI:
10.3791/2252-v

•

13:59 min

•

October 09, 2010

•

,
1Institute for Behavioral Genetics,University of Colorado, 2Integrative Physiology,University of Colorado

Chapters

  • 00:00Title
  • 00:21Subscription Link
  • 02:52Introduction
  • 03:31Preparing Nematoda Growth Media Plates for Paralysis Assay
  • 05:34Preparation of Age-synchronous Worm Populations
  • 08:04Transgene Induction and Paralysis Assay
  • 11:48Representative Results
  • 13:05Conclusion

Summary

Automatic Translation

Automatic Translation

强研究的线虫<em>线虫</em>可transgenically的设计，以表达人类β-淀粉样肽（Aβ）。诱导表达的Aβ<em> C。线虫</em>肌肉导致一个快速，重现性瘫痪的表型，可用于监测治疗，调节Aβ的毒性。

Tags

Assayingβ-amyloid ToxicityTransgenic C. Elegans ModelAlzheimer’s DiseaseToxicity MechanismInclusion Body MyositisNematode WormCaenorhabditis ElegansTransgenic EngineeringAβ ExpressionMeasurable PhenotypesAssociative LearningParalysis PhenotypeTemperature-sensitive MutationMRNA Surveillance SystemTemperature-inducible Muscle ExpressionAβ TransgeneProtective TransgeneGinkgo Biloba Extracts

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2252?article_type=v

含量自发活动，研究昼夜节律和睡眠参数果蝇</em
check_url/2252?article_type=v

线虫的积极丁酮学习，短期，长期联想记忆检测
check_url/2252?article_type=v

在 C 在体内激光干切断线虫
check_url/2252?article_type=v

突触荧光自动定量<em> C。线虫</em
check_url/2252?article_type=v

线虫跟踪和行为测量
check_url/2252?article_type=v

长期的嗅觉适应性的分子读数<em> C.线虫</em
check_url/2252?article_type=v

彗星试验测定DNA损伤的海马神经元
check_url/2252?article_type=v

C.在体内神经元钙成像技术线虫
check_url/2252?article_type=v

一<em>线虫</em>研究Amylopathy模型系统
check_url/2252?article_type=v

可视化的神经细胞胞质分裂过程中<em> C。线虫</em>胚胎

Read Article