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Crescita e la differenziazione degli adulti ippocampale suolo artico Cellule Staminali neurali Squirrel
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Chapters
- 00:05Title
- 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
- 01:05Preparing poly-L-ornithine-coated Flasks
- 01:56Thawing and Seeding agsNSC
- 03:39Expanding agsNSC
- 04:11Trypsinization of Cells
- 05:27Inoculating Well Plates for Differentiation
- 07:25Maintenance of Neurons
- 08:09Results of Growth and Differentiation of agsNSC
- 09:29Conclusion
Cellule staminali neurali sono stati preparati da ippocampo di adulti non-letargo yearling scoiattoli artici (AGS). Queste cellule staminali neurali può essere espansa attraverso numerosi passaggi, differenziate e mantenuto come un neurone quasi 50:50 alla cultura gliali.
Tags
GrowthDifferentiationAdult HippocampalArctic Ground SquirrelNeural Stem CellsHibernationIschemic InjuryBrainOxygen-glucose DeprivationNeuronsProteinsGene TargetsTherapiesIschemic IndicationsCold ToleranceProliferationCultureTUJ1-positive NeuronsToxic ChemicalsNeurogenesisMEK/ERK Signaling PathwayPI3K/Akt Signaling Pathway
