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Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
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Neurosciences
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Journal JoVE Neurosciences
Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells

Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells

DOI:
10.3791/2199-v

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09:44 min

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January 07, 2011

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, , ,
1Alaska Basic Neuroscience Program, Institute of Arctic Biology,University of Alaska at Fairbanks, 2Department Biochemistry,Hood College, 3Department of Cell Biology,Neuronascent, Inc., 4Research and Development,Neuronascent, Inc.

Chapitres

  • 00:05Titre
  • 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
  • 01:05Preparing poly-L-ornithine-coated Flasks
  • 01:56Thawing and Seeding agsNSC
  • 03:39Expanding agsNSC
  • 04:11Trypsinization of Cells
  • 05:27Inoculating Well Plates for Differentiation
  • 07:25Maintenance of Neurons
  • 08:09Results of Growth and Differentiation of agsNSC
  • 09:29Conclusion

Summary

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Neural stem cells were prepared from the hippocampus of adult non-hibernating yearling Arctic ground squirrels (AGS). These neural stem cells can be expanded through numerous passages, differentiated and maintained as a nearly 50:50 neuron to glial culture.

Tags

GrowthDifferentiationAdult HippocampalArctic Ground SquirrelNeural Stem CellsHibernationIschemic InjuryBrainOxygen-glucose DeprivationNeuronsProteinsGene TargetsTherapiesIschemic IndicationsCold ToleranceProliferationCultureTUJ1-positive NeuronsToxic ChemicalsNeurogenesisMEK/ERK Signaling PathwayPI3K/Akt Signaling Pathway

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