Neural stem cells were prepared from the hippocampus of adult non-hibernating yearling Arctic ground squirrels (AGS). These neural stem cells can be expanded through numerous passages, differentiated and maintained as a nearly 50:50 neuron to glial culture.
Drew, K. L., McGee, R. C., Wells, M. S., Kelleher-Andersson, J. A. Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells. J. Vis. Exp. (47), e2199, doi:10.3791/2199 (2011).