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Journal
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Biochemistry
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Separation och analys av submitokondriella vesiklar för att identifiera mitokondriella kontaktproteiner
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
Please note that all translations are automatically generated.
Click here for the English version.
Separation och analys av submitokondriella vesiklar för att identifiera mitokondriella kontaktproteiner
DOI:
10.3791/200432-v
•
05:03 min
•
June 16, 2023
•
Siavash Khosravi*
1,2
,
Johanna Frickel*
1
,
Max E. Harner
1
Department of Cell Biology, Biomedical Center, Medical Faculty
,
Ludwig-Maximilians University Munich
,
2
Cardio-Metabolic Diseases
,
Boehringer Ingelheim Pharma GmbH & Co. KG
Tags
– Submitochondrial Vesicles- Mitochondrial Contact Site Proteins- Centrifugation- Sucrose Cushion- Sucrose Gradient- Trichloroacetic Acid Precipitation- Protein Analysis- Mitochondria- Yeast- Homogenization- Refractometer- MOPS Buffer- EDTA- PMSF- Protease Inhibitors
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En förbättrad metod för att isolera mitokondriella kontaktställen
Generera submitokondriella vesiklar från
Saccharomyces cerevisiae
mitokondrier för att isolera mitokondriella kontaktställen
Separation och analys av submitokondriella vesiklar för att identifiera mitokondriella kontaktproteiner
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