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Biochemie
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Isolation of Mitochondrial Contact Sites
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Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
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Biochemie
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JoVE Journal Biochemie
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Isolation of Mitochondrial Contact Sites

DOI:
10.3791/200432-v

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05:03 min

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June 16, 2023

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, ,
1Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, 2Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Tags

– Submitochondrial Vesicles- Mitochondrial Contact Site Proteins- Centrifugation- Sucrose Cushion- Sucrose Gradient- Trichloroacetic Acid Precipitation- Protein Analysis- Mitochondria- Yeast- Homogenization- Refractometer- MOPS Buffer- EDTA- PMSF- Protease Inhibitors

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