Journal
/
Bioquímica
/
/
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

DOI:
10.3791/200432-v

•

05:03 min

•

June 16, 2023

•

, ,
1Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, 2Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Tags

– Submitochondrial Vesicles- Mitochondrial Contact Site Proteins- Centrifugation- Sucrose Cushion- Sucrose Gradient- Trichloroacetic Acid Precipitation- Protein Analysis- Mitochondria- Yeast- Homogenization- Refractometer- MOPS Buffer- EDTA- PMSF- Protease Inhibitors

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Generating Submitochondrial Vesicles from Saccharomyces cerevisiae Mitochondria to Isolate Mitochondrial Contact Sites

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Read Article