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Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

DOI:
10.3791/200432-v

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05:03 min

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June 16, 2023

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, ,
1Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, 2Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Tags

– Submitochondrial Vesicles- Mitochondrial Contact Site Proteins- Centrifugation- Sucrose Cushion- Sucrose Gradient- Trichloroacetic Acid Precipitation- Protein Analysis- Mitochondria- Yeast- Homogenization- Refractometer- MOPS Buffer- EDTA- PMSF- Protease Inhibitors

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