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Journal
/
Biochemistry
/
/
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins
DOI:
10.3791/200432-v
•
05:03 min
•
June 16, 2023
•
Siavash Khosravi*
1,2
,
Johanna Frickel*
1
,
Max E. Harner
1
Department of Cell Biology, Biomedical Center, Medical Faculty
,
Ludwig-Maximilians University Munich
,
2
Cardio-Metabolic Diseases
,
Boehringer Ingelheim Pharma GmbH & Co. KG
Tags
– Submitochondrial Vesicles- Mitochondrial Contact Site Proteins- Centrifugation- Sucrose Cushion- Sucrose Gradient- Trichloroacetic Acid Precipitation- Protein Analysis- Mitochondria- Yeast- Homogenization- Refractometer- MOPS Buffer- EDTA- PMSF- Protease Inhibitors
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