Journal
/
Biochemistry
/
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
13C 4-الأسبارتات و 13C5-الجلوتامين تتبع النظائر المستقرة ، تحليل LC-MS ، وقياس نشاط SDH
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement
Please note that all translations are automatically generated. Click here for the English version.

13C 4-الأسبارتات و 13C5-الجلوتامين تتبع النظائر المستقرة ، تحليل LC-MS ، وقياس نشاط SDH

العربية

Automatically Generated

292 Views

•

04:55 min

•

May 19, 2023

DOI:

10.3791/200017-v

DOI:
10.3791/200017-v

•

04:55 min
May 19, 2023

•

5 Views
, , ,
1Program in Molecular Medicine,University of Massachusetts Chan Medical School, 2Cancer Center,University of Massachusetts Chan Medical School

Explore More Videos

13C4-aspartate13C5-glutamineStable Isotope TracingLC-MS AnalysisSDH Activity Measurement143B Human Osteosarcoma CellsCell CultureMetabolite ExtractionVacuum ConcentrationLiquid ChromatographyMass Spectrometry

Read Article

Cite this Article

Tseyang, T., Valeros, J., Vo, P., Spinelli, J. B. 13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement. J. Vis. Exp. (Pending Publication), e200017, doi:10.3791/200017 (2023).

Download .ris file

Copy

Share Video

.

Copy