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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement
JoVE Journal
Biochemie
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JoVE Journal Biochemie
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

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04:55 min

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May 19, 2023

DOI:

10.3791/200017-v

DOI:
10.3791/200017-v

•

04:55 min
May 19, 2023

•

5 Views
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1Program in Molecular Medicine,University of Massachusetts Chan Medical School, 2Cancer Center,University of Massachusetts Chan Medical School

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Tseyang, T., Valeros, J., Vo, P., Spinelli, J. B. 13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement. J. Vis. Exp. (Pending Publication), e200017, doi:10.3791/200017 (2023).

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