Journal
/
Biochemie
/
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal Biochemie
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

231 Views

•

04:55 min

•

May 19, 2023

DOI:

10.3791/200017-v

DOI:
10.3791/200017-v

•

04:55 min
May 19, 2023

•

5 Views
, , ,
1Program in Molecular Medicine,University of Massachusetts Chan Medical School, 2Cancer Center,University of Massachusetts Chan Medical School

Explore More Videos

13C4-aspartate13C5-glutamineStable Isotope TracingLC-MS AnalysisSDH Activity Measurement143B Human Osteosarcoma CellsCell CultureMetabolite ExtractionVacuum ConcentrationLiquid ChromatographyMass Spectrometry

Read Article

Cite this Article

Tseyang, T., Valeros, J., Vo, P., Spinelli, J. B. 13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement. J. Vis. Exp. (Pending Publication), e200017, doi:10.3791/200017 (2023).

Download .ris file

Copy

Share Video

.

Copy