Journal
/
Biochemistry
/
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

DOI:
10.3791/200017-v

•

04:55 min

•

May 19, 2023

•

, , ,
1Program in Molecular Medicine,University of Massachusetts Chan Medical School, 2Cancer Center,University of Massachusetts Chan Medical School

Tags

Keywords: 13C4-aspartate13C5-glutamineStable Isotope TracingLC-MS AnalysisSDH Activity Measurement143B Human Osteosarcoma CellsCell CultureMetabolite ExtractionVacuum ConcentrationLiquid ChromatographyMass Spectrometry

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/fr/200017?article_type=v

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals (Video) | JoVE

check_url/fr/200017?article_type=v

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

Read Article