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Biochemistry

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JoVE Journal Biochemistry
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE
 

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

Article DOI: 10.3791/200017-v • 04:55 min • May 19th, 2023
May 19th, 2023 •
*1, *1, *1, 1,2
1Program in Molecular Medicine, University of Massachusetts Chan Medical School, 2Cancer Center, University of Massachusetts Chan Medical School
* These authors contributed equally

Tags

Oxygen-independent Assays Mitochondrial Function Mammals Metabolic Stressors Hypoxia Low-oxygen Environments Higher Resolution Mass Spectrometry Stable Isotope Tracing Studies Metabolite Levels Alternative Electron Acceptor Electron Transport Chain (ETC) Fumarate Mitochondrial Function Assessment
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