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Entwicklung von automatisierten Imaging und Analyse für Zebrafisch chemische Bildschirme.
JoVE Journal
Biology
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JoVE Journal Biology
Development of automated imaging and analysis for zebrafish chemical screens.
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Entwicklung von automatisierten Imaging und Analyse für Zebrafisch chemische Bildschirme.

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11,146 Views

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10:49 min

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June 24, 2010

DOI:

10.3791/1900-v

DOI:
10.3791/1900-v

•

10:49 min
June 24, 2010

•

1 Views
, , , ,
1Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh, 3Department of Pharmaceutical Sciences,University of Pittsburgh, 4Department of Chemistry,University of Pittsburgh

Summary

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Wir berichten über die Entwicklung eines Systems zur automatisierten Abbildung und Analyse von Zebrafisch-transgenen Embryos in Mikrotiterplatten. Dies demonstriert die Fähigkeit, dosisabhängige Wirkung eines kleinen Moleküls, BCI, auf Fibroblast Growth Factor Expression des Reportergens zu messen und Technologie für den Aufbau High-Throughput-Zebrafisch chemische Bildschirme.

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Vogt, A., Codore, H., Day, B. W., Hukriede, N. A., Tsang, M. Development of automated imaging and analysis for zebrafish chemical screens.. J. Vis. Exp. (40), e1900, doi:10.3791/1900 (2010).

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