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Development of automated imaging and analysis for zebrafish chemical screens.
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Development of automated imaging and analysis for zebrafish chemical screens.

Development of automated imaging and analysis for zebrafish chemical screens.

11,146 Views

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10:49 min

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June 24, 2010

DOI:

10.3791/1900-v

DOI:
10.3791/1900-v

•

10:49 min
June 24, 2010

•

1 Views
, , , ,
1Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh, 3Department of Pharmaceutical Sciences,University of Pittsburgh, 4Kimya Bölümü,University of Pittsburgh

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We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

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Vogt, A., Codore, H., Day, B. W., Hukriede, N. A., Tsang, M. Development of automated imaging and analysis for zebrafish chemical screens.. J. Vis. Exp. (40), e1900, doi:10.3791/1900 (2010).

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