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Biology

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Development of automated imaging and analysis for zebrafish chemical screens.
 

Development of automated imaging and analysis for zebrafish chemical screens.

Article DOI: 10.3791/1900-v • 10:50 min • June 24th, 2010
June 24th, 2010 •
1, 2, 3,4, 2, 2
1Pharmacology and Chemical Biology, University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics, University of Pittsburgh, 3Department of Pharmaceutical Sciences, University of Pittsburgh, 4Department of Chemistry, University of Pittsburgh

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We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

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Automated Imaging Analysis Zebrafish Chemical Screens Image-based High-content Screening FGF/RAS/MAPK Pathway Zebrafish Embryos In Vivo High-content Chemical Screens 96-well Plates High Throughput Screening Automated Image Acquisition And Analysis Transgenic Reporter Line Green Fluorescent Protein (GFP) Whole Organism Screens Automated Imaging And Analysis 24-48 Hours Post Fertilization (hpf) Multiwell Plates Small Molecule Hyperactivates FGF Signaling
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