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Development of automated imaging and analysis for zebrafish chemical screens.
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生物学
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JoVE Journal 生物学
Development of automated imaging and analysis for zebrafish chemical screens.

Development of automated imaging and analysis for zebrafish chemical screens.

11,146 Views

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10:49 min

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June 24, 2010

DOI:

10.3791/1900-v

DOI:
10.3791/1900-v

•

10:49 min
June 24, 2010

•

1 Views
, , , ,
1Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh, 3Department of Pharmaceutical Sciences,University of Pittsburgh, 4化学科,University of Pittsburgh

概要

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We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

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Cite this Article

Vogt, A., Codore, H., Day, B. W., Hukriede, N. A., Tsang, M. Development of automated imaging and analysis for zebrafish chemical screens.. J. Vis. Exp. (40), e1900, doi:10.3791/1900 (2010).

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