/
/
Magnetic Resonance Spectroscopy von Live- Drosophila melanogaster Verwendung von Magic Angle Spinning
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1NMR Surgical Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School, 2Shriners Burn Institute, 3Department of Radiology, Athinoula A. Martinos Center of Biomedical Imaging,Harvard Medical School, 4Molecular Surgery Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School
Chapters
- 00:00Title
- 00:43Introduction
- 01:53Preparing Drosophila for HRMAS Measurements
- 02:57HRMAS Rotor Preparation
- 03:58HRMAS Data Acquisition
- 05:27Data Processing/ Analysis
- 06:05Representative Results/ Outcome
- 06:50Conclusion
Diese Technik ermöglicht den Einsatz von hochauflösenden magic angle spinning Protonen MR-Spektroskopie (HR-MAS-1H-MRS) für die molekulare Charakterisierung von Live-<em> Drosophila melanogaster</em> Mit einem herkömmlichen 14,1-Tesla-Spektrometer mit einer HR-MAS-Sonde ausgestattet.
Tags
Magnetic Resonance SpectroscopyLive Drosophila MelanogasterMagic Angle SpinningHigh-Resolution Magic Angle SpinningHRMAS Proton Magnetic Resonance Spectroscopy1H-MRSNon-destructive TechniqueSpectral Line-widthsHigh-resolution SpectraMetabolitesCellular ProcessesIn Vivo StudiesFruit Fly Drosophila MelanogasterGenetic OrganismBiological FunctionsSignaling PathwaysGenetics And PhysiologyIn Vivo HRMAS 1H-MRS Method14.1 TMR SpectrometerHRMAS ProbeMolecular CharacterizationDisease BiomarkersTherapeutic Development
