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Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
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神经科学
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JoVE 杂志 神经科学
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

DOI:
10.3791/1710-v

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07:33 min

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April 15, 2010

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, , ,
1NMR Surgical Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School, 2Shriners Burn Institute, 3Department of Radiology, Athinoula A. Martinos Center of Biomedical Imaging,Harvard Medical School, 4Molecular Surgery Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School

Chapters

  • 00:00Title
  • 00:43Introduction
  • 01:53Preparing Drosophila for HRMAS Measurements
  • 02:57HRMAS Rotor Preparation
  • 03:58HRMAS Data Acquisition
  • 05:27Data Processing/ Analysis
  • 06:05Representative Results/ Outcome
  • 06:50Conclusion

Summary

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This technique enables the use of high-resolution magic angle spinning proton MR spectroscopy (HRMAS 1H-MRS) for molecular characterization of live Drosophila melanogaster with a conventional 14.1 tesla spectrometer equipped with an HRMAS probe.

Tags

Magnetic Resonance SpectroscopyLive Drosophila MelanogasterMagic Angle SpinningHigh-Resolution Magic Angle SpinningHRMAS Proton Magnetic Resonance Spectroscopy1H-MRSNon-destructive TechniqueSpectral Line-widthsHigh-resolution SpectraMetabolitesCellular ProcessesIn Vivo StudiesFruit Fly Drosophila MelanogasterGenetic OrganismBiological FunctionsSignaling PathwaysGenetics And PhysiologyIn Vivo HRMAS 1H-MRS Method14.1 TMR SpectrometerHRMAS ProbeMolecular CharacterizationDisease BiomarkersTherapeutic Development

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