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Spectroscopie par résonance magnétique du direct Drosophila melanogaster En utilisant Magic Angle Spinning
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07:33 min
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April 15, 2010
DOI:
1NMR Surgical Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School, 2Shriners Burn Institute, 3Department of Radiology, Athinoula A. Martinos Center of Biomedical Imaging,Harvard Medical School, 4Molecular Surgery Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School
Summary
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Cette technique permet l'utilisation de l'angle magique à haute résolution filer spectroscopie par résonance magnétique protonique (HRMAS 1H-MRS) pour la caractérisation moléculaire de vivre<em> Drosophila melanogaster</em> Avec un spectromètre de 14,1 teslas conventionnelle équipée d'une sonde HRMAS.
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Magnetic Resonance SpectroscopyLive Drosophila MelanogasterMagic Angle SpinningHigh-Resolution Magic Angle SpinningHRMAS Proton Magnetic Resonance Spectroscopy1H-MRSNon-destructive TechniqueSpectral Line-widthsHigh-resolution SpectraMetabolitesCellular ProcessesIn Vivo StudiesFruit Fly Drosophila MelanogasterGenetic OrganismBiological FunctionsSignaling PathwaysGenetics And PhysiologyIn Vivo HRMAS 1H-MRS Method14.1 TMR SpectrometerHRMAS ProbeMolecular CharacterizationDisease BiomarkersTherapeutic Development
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Cite this Article
Righi, V., Apidianakis, Y., Rahme, L. G., Tzika, A. A. Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning. J. Vis. Exp. (38), e1710, doi:10.3791/1710 (2010).
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