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A High-Throughput Metodo per la crioconservazione dello sperma e Zebrafish In Vitro Fecondazione
JoVE Journal
Biology
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JoVE Journal Biology
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
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A High-Throughput Metodo per la crioconservazione dello sperma e Zebrafish In Vitro Fecondazione

italiano

Automatically Generated

30,482 Views

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11:01 min

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July 06, 2009

DOI:

10.3791/1395-v

DOI:
10.3791/1395-v

•

11:01 min
July 06, 2009

•

17 Views
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1Molecular and Cellular Biology,University of California, Davis, 2Division of Basic Science,Fred Hutchinson Cancer Research Center – FHCRC

Summary

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Si tratta di un high-throughput protocollo di crioconservazione degli spermatozoi per zebrafish. Spermatozoi crioconservati utilizzando questo protocollo ha una media del 25% la fertilità fertilizzazione in vitro successive ed è stabile per molti anni.

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Cite this Article

Draper, B. W., Moens, C. B. A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization. J. Vis. Exp. (29), e1395, doi:10.3791/1395 (2009).

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