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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Journal JoVE
Biologie
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Journal JoVE Biologie
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

DOI:
10.3791/1395-v

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11:01 min

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July 06, 2009

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,
1Molecular and Cellular Biology,University of California, Davis, 2Division of Basic Science,Fred Hutchinson Cancer Research Center – FHCRC

Chapitres

  • 00:05Titre
  • 00:08Introduction
  • 01:02Sperm Freezing Solutions and Materials
  • 02:04Sperm Freezing Method
  • 06:50In Vitro Fertilization Method Using Cryopreserved Sperm
  • 09:31Representative Results/ Outcome
  • 09:57Conclusion

Summary

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Traduction automatique

This is a high-throughput sperm cryopreservation protocol for zebrafish. Sperm cryopreserved using this protocol has an average of 25% fertility in subsequent vitro fertilization and is stable over many years.

Tags

Zebrafish Sperm CryopreservationIn Vitro FertilizationHigh-throughput MethodHarvey Method AdaptationSample UniformityFreezing MediaCryoprotectantCryovialsSperm FreezingSperm ThawingCapillary TubesTeam WorkFertility Rate

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