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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
JoVE Journal
Biologia
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JoVE Journal Biologia
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

30,484 Views

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11:01 min

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July 06, 2009

DOI:

10.3791/1395-v

DOI:
10.3791/1395-v

•

11:01 min
July 06, 2009

•

17 Views
,
1Molecular and Cellular Biology,University of California, Davis, 2Division of Basic Science,Fred Hutchinson Cancer Research Center – FHCRC

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This is a high-throughput sperm cryopreservation protocol for zebrafish. Sperm cryopreserved using this protocol has an average of 25% fertility in subsequent vitro fertilization and is stable over many years.

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Cite this Article

Draper, B. W., Moens, C. B. A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization. J. Vis. Exp. (29), e1395, doi:10.3791/1395 (2009).

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