Journal
/
Biology
/
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

English

Automatically Generated

30,484 Views

•

11:01 min

•

July 06, 2009

DOI:

10.3791/1395-v

DOI:
10.3791/1395-v

•

11:01 min
July 06, 2009

•

17 Views
,
1Molecular and Cellular Biology,University of California, Davis, 2Division of Basic Science,Fred Hutchinson Cancer Research Center – FHCRC

Summary

Automatically generated

This is a high-throughput sperm cryopreservation protocol for zebrafish. Sperm cryopreserved using this protocol has an average of 25% fertility in subsequent vitro fertilization and is stable over many years.

Explore More Videos

Zebrafish Sperm CryopreservationIn Vitro FertilizationHigh-throughput MethodHarvey Method AdaptationSample UniformityFreezing MediaCryoprotectantCryovialsSperm FreezingSperm ThawingCapillary TubesTeam WorkFertility Rate

Read Article

Cite this Article

Draper, B. W., Moens, C. B. A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization. J. Vis. Exp. (29), e1395, doi:10.3791/1395 (2009).

Download .ris file

Copy

Share Video

.

Copy