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Preparazione di proiettili pistola Gene e Biolistic Transfection dei neuroni in cultura Slice
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
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Preparazione di proiettili pistola Gene e Biolistic Transfection dei neuroni in cultura Slice

52,001 Views

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17:42 min

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February 13, 2008

DOI:

10.3791/675-v

DOI:
10.3791/675-v

•

17:42 min
February 13, 2008

•

16 Views
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1Center for Neuroscience,University of California, Davis

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Descriviamo un metodo per la preparazione di proiettili rivestiti d'oro del DNA e dimostrare l'uso di proiettili ad esempio per trasfettare biolistically neuroni in colture di fettine di ippocampo.

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Cite this Article

Woods, G., Zito, K. Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture. J. Vis. Exp. (12), e675, doi:10.3791/675 (2008).

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