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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

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12:14 min

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September 14, 2022

DOI:

10.3791/64574-v

DOI:
10.3791/64574-v

•

12:14 min
September 14, 2022

•

59 Views
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1Department of Neurology,Yale University, 2Nörobilim Bölümü,Yale University, 3Interdepartmental Neuroscience Program,Yale University

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This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

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Massaro Tieze, S., Chandra, S. S., Vidyadhara, D. J. Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain. J. Vis. Exp. (187), e64574, doi:10.3791/64574 (2022).

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