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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
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神経科学
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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

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12:14 min

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September 14, 2022

DOI:

10.3791/64574-v

DOI:
10.3791/64574-v

•

12:14 min
September 14, 2022

•

59 Views
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1Department of Neurology,Yale University, 2神経科学科,Yale University, 3Interdepartmental Neuroscience Program,Yale University

概要

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This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

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Cite this Article

Massaro Tieze, S., Chandra, S. S., Vidyadhara, D. J. Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain. J. Vis. Exp. (187), e64574, doi:10.3791/64574 (2022).

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