Journal
/
Neurociencias
/
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
JoVE Revista
Neurociencias
This content is Free Access.
JoVE Revista Neurociencias
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

4,500 Views

•

12:14 min

•

September 14, 2022

DOI:

10.3791/64574-v

DOI:
10.3791/64574-v

•

12:14 min
September 14, 2022

•

59 Views
, ,
1Department of Neurology,Yale University, 2Department of Neuroscience,Yale University, 3Interdepartmental Neuroscience Program,Yale University

Summary

Automatically generated

This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

Explore More Videos

Subcellular FractionationSynapsesSynaptosomeBrain DisordersProtein DistributionProtein ActivityBuffer Preparation

Read Article

Cite this Article

Massaro Tieze, S., Chandra, S. S., Vidyadhara, D. J. Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain. J. Vis. Exp. (187), e64574, doi:10.3791/64574 (2022).

Download .ris file

Copy

Share Video

.

Copy