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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

4,574 Views

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12:14 min

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September 14, 2022

DOI:

10.3791/64574-v

DOI:
10.3791/64574-v

•

12:14 min
September 14, 2022

•

59 Views
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1Department of Neurology,Yale University, 2Department of Neuroscience,Yale University, 3Interdepartmental Neuroscience Program,Yale University

Summary

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This protocol presents a robust, detailed method to obtain highly pure synaptosomes, synaptic vesicles, and other synaptic fractions from the mouse brain. This method enables the evaluation of synaptic processes, including the biochemical analysis of protein localization and function with compartmental resolution.

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Cite this Article

Massaro Tieze, S., Chandra, S. S., Vidyadhara, D. J. Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain. J. Vis. Exp. (187), e64574, doi:10.3791/64574 (2022).

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