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使用MALDI-TOF质谱法鉴定酶降解产生的RNA片段
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Biyokimya
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JoVE Journal Biyokimya
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
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使用MALDI-TOF质谱法鉴定酶降解产生的RNA片段

DOI:
10.3791/63720-v

•

09:20 min

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April 11, 2022

•

, , , ,
1Kimya Bölümü,University of Colorado, Denver, 2Department of Chemistry & Biochemistry,University of Denver, 3Analytical Resources Core Bioanalysis & Omics,Colorado State University

Bölümler

  • 00:09Introduction
  • 01:05Concentration Determination of RNA Solution
  • 03:15Hydrolysis of Oligonucleotides of RNA by Xrn-1
  • 05:31Desalting of RNA Solution, MALDI-TOF Plate Spotting
  • 07:41Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry
  • 08:32Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

MALDI-TOF用于表征从氧化RNA和外切核糖核酸酶Xrn-1之间的反应性中获得的片段。本方案描述了一种可应用于涉及RNA和/或DNA的其他过程的方法。

Etiketler

RNA FragmentsEnzymatic DegradationMALDI-TOF Mass SpectrometryBiopolymer CharacterizationBiochemical TransformationsUV-Vis SpectrophotometryPeltier Temperature Control

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