Dergi
/
Nörobilim
/
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

DOI:
10.3791/61110-v

•

06:47 min

•

May 21, 2020

•

, , , , , , , , , ,
1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris

Bölümler

  • 00:04Introduction
  • 00:43Surgical Preparation
  • 01:39Pregnant Mouse Preparation
  • 02:16Intraventricular Injection
  • 03:16In Utero Electroporation (IUE)
  • 04:24Multichannel Confocal Imaging
  • 05:23Results: Representative Cortical Astrocyte Segmentation and Arborization
  • 06:10Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.

Etiketler

In Utero ElectroporationMAGIC MarkersCortical AstrocytesIndividualizationMorphology AssessmentProgenitor Cell LabelingNeuronal And Viral ProgenyMicropipetteDNA SolutionLateral Ventricle InjectionEmbryonic Day 15.5Surgical Procedure

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Isolation and Culture of Mouse Cortical Astrocytes

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Subtype-selective Electroporation of Cortical Interneurons

Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse

Read Article