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In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris
Chapters
- 00:04Introduction
- 00:43Surgical Preparation
- 01:39Pregnant Mouse Preparation
- 02:16Intraventricular Injection
- 03:16In Utero Electroporation (IUE)
- 04:24Multichannel Confocal Imaging
- 05:23Results: Representative Cortical Astrocyte Segmentation and Arborization
- 06:10Conclusion
Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.
