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In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
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In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
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In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

5,201 Views

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06:47 min

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May 21, 2020

DOI:

10.3791/61110-v

DOI:
10.3791/61110-v

•

06:47 min
May 21, 2020

•

8 Views
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1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris

Özet

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Gli astrociti piastrellano uniformemente la corteccia cerebrale, rendendo difficile l'analisi della loro complessa morfologia a livello cellulare. Il protocollo qui fornito utilizza l'etichettatura multicolore basata sull'elettroporazione in utero per singolo astrociti corticali e analizzarne il volume e la morfologia con una pipeline di analisi delle immagini intuitiva.

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Dumas, L., Clavreul, S., Durand, J., Hernandez-Garzon, E., Abdeladim, L., Barry-Martinet, R., Caballero-Megido, A., Beaurepaire, E., Bonvento, G., Livet, J., Loulier, K. In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes. J. Vis. Exp. (159), e61110, doi:10.3791/61110 (2020).

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