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在多地址基因组集成颜色 （MAGIC） 标记的 Utero 电冲中， 以个性化皮质小鼠星形细胞
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May 21, 2020
DOI:
1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris
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星形细胞均匀地平铺大脑皮层，使得对其复杂形态的分析在细胞水平上具有挑战性。此处提供的协议使用基于子宫电镀的多色标签，挑出皮质星形细胞，并使用用户友好的图像分析管道分析其体积和形态。
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Dumas, L., Clavreul, S., Durand, J., Hernandez-Garzon, E., Abdeladim, L., Barry-Martinet, R., Caballero-Megido, A., Beaurepaire, E., Bonvento, G., Livet, J., Loulier, K. In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes. J. Vis. Exp. (159), e61110, doi:10.3791/61110 (2020).
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