Dergi
/
Kanser Araştırmaları
/
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
JoVE Journal
Kanser Araştırmaları
This content is Free Access.
JoVE Journal Kanser Araştırmaları
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

DOI:
10.3791/60316-v

•

09:29 min

•

March 20, 2020

•

, , , , , , ,
1Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research,National Cancer Institute at Frederick, 2Small Animal Imaging Program, Frederick National Laboratory for Cancer Research,Leidos Biomedical Research, Inc.

Bölümler

  • 00:05Introduction
  • 00:55Orthotopic Breast Cancer Model and Tumor Size Measurement
  • 02:05In Vivo Bioluminescence and Ex Vivo Fluorescence Imaging
  • 04:19Molecular Metastatic Breast Cancer Cell Detection
  • 06:27Lung Metastatic Breast Cancer Cell Detection
  • 07:29Results: Representative Bioluminescence Imaging (BLI) and GFP Signal Detection
  • 08:52Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.

Etiketler

Triple Negative Breast CancerOrthotopic Breast Cancer ModelGFP TagBioluminescence ImagingMicrometastasisPCR AnalysisAnti-cancer AgentsEMTDrug ResistanceCancer Stem Cell MechanismsMetastatic ProcessAnti-cancer Drug DevelopmentXenograftTumor Size MeasurementLuciferin KineticsLung MetastasisBrain Metastasis

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

Ultrasonographic Evaluation of Breast Cancer-related Lymphedema

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

Read Article