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癌症研究
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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

16,347 Views

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09:29 min

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March 20, 2020

DOI:

10.3791/60316-v

DOI:
10.3791/60316-v

•

09:29 min
March 20, 2020

•

20 Views
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1Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research,National Cancer Institute at Frederick, 2Small Animal Imaging Program, Frederick National Laboratory for Cancer Research,Leidos Biomedical Research, Inc.

Summary

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This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.

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Triple Negative Breast CancerOrthotopic Breast Cancer ModelGFP TagBioluminescence ImagingMicrometastasisPCR AnalysisAnti-cancer AgentsEMTDrug ResistanceCancer Stem Cell MechanismsMetastatic ProcessAnti-cancer Drug DevelopmentXenograftTumor Size MeasurementLuciferin KineticsLung MetastasisBrain Metastasis

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Cite this Article

Cheng, R. Y. S., Patel, N. L., Back, T., Basudhar, D., Somasundaram, V., Kalen, J. D., Wink, D. A., Ridnour, L. A. Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model. J. Vis. Exp. (157), e60316, doi:10.3791/60316 (2020).

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