This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.
Cheng, R. Y. S., Patel, N. L., Back, T., Basudhar, D., Somasundaram, V., Kalen, J. D., Wink, D. A., Ridnour, L. A. Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model. J. Vis. Exp. (157), e60316, doi:10.3791/60316 (2020).