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Cancer Research

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JoVE Journal Cancer Research
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
 

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Article DOI: 10.3791/60316-v • 09:29 min • March 20th, 2020
March 20th, 2020 •
1, 2, 1, 1, 1, 2, 1, 1
1Molecular Mechanism Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, 2Small Animal Imaging Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc.

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This work presets an advanced protocol to accurately assess tumor loading by detection of green fluorescent protein and bioluminescence signals as well as the integration of quantitative molecular detection technique.

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Triple Negative Breast Cancer Orthotopic Breast Cancer Model Bioluminescence Imaging Window GFP Tag Micrometastasis PCR Analysis Therapeutic Efficacy Anti-cancer Agents EMT Drug Resistance Cancer Stem Cell Mechanisms Metastatic Process Anti-cancer Drug Development Pedal Reflex Mammary Gland Rat-tooth Forceps 25 Gauge Needle One Milliliter Syringe Cell Basement Membrane Matrix Mix
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