Dergi
/
Nörobilim
/
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

25,245 Views

•

05:25 min

•

July 01, 2019

DOI:

10.3791/59683-v

DOI:
10.3791/59683-v

•

05:25 min
July 01, 2019

•

63 Views
, , ,
1Biyomedikal Bilimler bölümü,University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Özet

Automatically generated

This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.

Explore More Videos

Mouse RetinaRetinal DissectionImmunohistochemistryCryo-sectionEye EnucleationEye FixationCornea RemovalLens ExtractionRetina IsolationCryo-protectionSnap Freezing

Read Article

Cite this Article

Léger, H., Santana, E., Beltran, W. A., Luca, F. C. Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry. J. Vis. Exp. (149), e59683, doi:10.3791/59683 (2019).

Download .ris file

Copy

Share Video

.

Copy