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Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
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JoVE Journal Neuroscienze
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

25,245 Views

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05:25 min

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July 01, 2019

DOI:

10.3791/59683-v

DOI:
10.3791/59683-v

•

05:25 min
July 01, 2019

•

63 Views
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1Department of Biomedical Sciences,University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Summary

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This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.

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Cite this Article

Léger, H., Santana, E., Beltran, W. A., Luca, F. C. Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry. J. Vis. Exp. (149), e59683, doi:10.3791/59683 (2019).

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