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Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
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JoVE Journal 神経科学
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

25,242 Views

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05:25 min

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July 01, 2019

DOI:

10.3791/59683-v

DOI:
10.3791/59683-v

•

05:25 min
July 01, 2019

•

63 Views
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1生物医科学科,University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

概要

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This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.

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Cite this Article

Léger, H., Santana, E., Beltran, W. A., Luca, F. C. Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry. J. Vis. Exp. (149), e59683, doi:10.3791/59683 (2019).

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