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Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
Chapters
- 00:04Title
- 00:45Mouse Eye Enucleation, Eye Cup Dissection, and Embedding
- 03:08Sectioning Using a Cryostat
- 03:51Results: Immunohistochemistry of Mouse Retinal Cryo-sections
- 05:01Conclusion
This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.
