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全山荧光在小鼠胚胎发生过程中心脏室发育的分析
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
Please note that all translations are automatically generated. Click here for the English version.

全山荧光在小鼠胚胎发生过程中心脏室发育的分析

7,295 Views

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06:27 min

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April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Özet

Automatically generated

我们提出的方案, 以检查小鼠心脏的发展使用整个安装视网膜显微镜从心室特异性 Mlc-2v-t分解番茄记者敲入小鼠解剖小鼠胚胎。这种方法使我们能够直接想象小鼠心脏发育过程中心室形成的每个阶段, 而无需劳动密集型组织化学方法。

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Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

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