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Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
JoVE Journal
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JoVE Journal Nörobilim
Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

DOI:
10.3791/59232-v

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08:52 min

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May 23, 2019

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, , ,
1Departments of Bioengineering,Washington Üniversitesi, 2Washington National Primate Research Center, 3Elektrik ve Bilgisayar Mühendisliği Bölümü,Washington Üniversitesi, 4Department of Physiology and Center for Integrative Neuroscience,University of California, San Francisco

Bölümler

  • 00:04Başlık
  • 00:39MR-compatible Chamber Implantation
  • 02:05Viral Vector Delivery
  • 06:18Results: Validation of Viral Expression
  • 07:47Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

Etiketler

Convection Enhanced DeliveryOptogeneticAdeno-associated Viral VectorCortexRhesus MacaqueMRICraniotomyDuraMR Compatible ChamberInjection GridSalineSterile Absorbable Gel FoamVitamin E Capsule

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